Medium chain Fatty acids production from Food Waste via homolactic fermentation and lactate/ethanol elongation: Electron balance and thermodynamic assessment.

This study explored the potential of Food Waste (FW) extract as a suitable substrate for Medium Chain Fatty Acids (MCFAs) production, in a single-phase reactor, where both fermentation and Chain Elongation (CE) processes occurred simultaneously. A continuous experiment was conducted with an Organic Loading Rate (OLR) = 20 gCOD L-1 d-1 and was fed in batch mode twice a week with pH = 6. In addition, four batch tests were performed, to assess the effects on the MCFAs production of caproate inhibition, hydrogen partial pressure (PH2) and different lactate/acetate ratios. Thermodynamics and electron flux were calculated to gain insights into the process pathways. Due to the presence of aminoacids, fermentation was mostly homolactic and both lactate and ethanol were produced as Electron Donors (EDs); the average MCFAs production efficiency was âˆ¼ 12 %, although after 4 weeks the elongation process was halted, resulting in EDs accumulation. This occurred regardless of inoculum selection and the presence of caproate as a possible inhibitor, suggesting that EDs accumulation was due to the elongation process kinetics being slower than those of the fermentation step, thus calling for a longer Hydraulic Retention Time (HRT). It's worth noting that lactate was prevalently self-elongated to butyrate, whereas ethanol elongation only took place after lactate depletion, but was more efficient since it required other Electron Acceptors (EAs) such as butyrate, propionate or valerate. Moreover, the selected pH limited the acrylate pathway to a reasonable extent, whereas the high PH2 prevented both ethanol and lactate oxydation to acetate.

Thermally enhanced solid-liquid separation process in food waste biorefinery: modelling the anaerobic digestion of solid residues.

The biochemical valorization potential of food waste (FW) could be exploited by extracting decreasing added-value bio-based products and converting the final residues into energy. In this context, multi-purpose and versatile schemes integrating thermal and biochemical conversion processes will play a key role. An upstream thermal pretreatment + solid-liquid separation unit was here proposed to optimize the conversion of the liquid fraction of FW into valuable chemicals through semi-continuous fermentation process, and the conversion of the residual solid fraction into biomethane through anaerobic digestion. The solid residues obtained after thermal pretreatment presented a higher soluble COD fraction, which resulted in higher methane production with respect to the raw residues (0.33 vs. 0.29 Nm3CH4 kg-1VSfed) and higher risk of acidification and failure of methanogenesis when operating at lower HRT (20d). On the contrary, at HRT = 40 d, the pretreatment did not affect the methane conversion rates and both tests evidenced similar methane productions of 0.33 Nm3CH4 kg-1VSfed. In the reactor fed with pretreated residue, the association of hydrogenotrophic methanogens with syntrophic bacteria prevented the acidification of the system. Modelling proved the eligibility of the FW solid residues as substrates for anaerobic digestion, given their small inert fractions that ranged between 0% and 30% of the total COD content.

Direct Conversion of Food Waste Extract into Caproate: Metagenomics Assessment of Chain Elongation Process.

In a circular economy strategy, waste resources can be used for the biological production of high added-value substances, such as medium chain fatty acids (MCFAs), thus minimising waste and favouring a sustainable process. This study investigates single-stage fermentation processes for the production of MCFAs in a semi-continuous reactor treating the extract of real food waste (FW), without the addition of external electron donors. Two sequential acidogenic fermentation tests were carried out at an organic loading rate (OLR) of 5 and 15 gCOD L-1d-1 with a hydraulic retention time of 4 days and pH controlled at 6 ± 0.2. The highest level of caproate (4.8 g L-1) was observed at OLR of 15 gCOD L-1d-1 with a microbiome mainly composed by lactate-producing Actinomyces, Atopobium, and Olsenella species and caproate-producing Pseudoramibacter. Metagenomic analysis revealed the presence of key enzymes for the production of lactate, such as lactate dehydrogenase and pyruvate ferredoxin oxidoreductase, as well as several enzymes involved in the reverse ß-oxidation pathway, thus suggesting the occurrence of a lactate-based chain elongation process.

Kitchen waste valorization through a mild-temperature pretreatment to enhance biogas production and fermentability: Kinetics study in mesophilic and thermophilic regimen.

Biowaste valorization through anaerobic digestion is an attractive option to achieve both climate protection goals and renewable energy production. In this paper, a complete set of batch trials was carried out on kitchen waste to investigate the effects of mild thermal pretreatment, temperature regimen and substrate/inoculum ratio. Thermal pretreatment was effective in the solubilisation of macromolecular fractions, particularly carbohydrates. The ability of the theoretical methodologies in estimating hydrogen and methane yields of complex substrates was evaluated by comparing the experimental results with the theoretical values. Despite the single batch configuration, a significant initial hydrogen production was observed, prior to methane yield. Main pretreatment effect was the gain in hydrogen production; the extent was highly variable according to the other parameters values. High hydrogen yields, up to 113 mL H2/g VSfed, were related to the prompt transformation of soluble sugars. Thermophilic regimen resulted, as expected, in faster digestions (up to 78 mL CH4/gVS/day) and sorted out pH inhibition. The relatively low methane yields (342-398 mL CH4/g VSfed) were the result of the consistent lignocellulosic content and low lipid content. Thermal pretreatment proved to be a promising option for the enhancement of hydrogen production in food waste dark fermentation.

Anaerobic digestion of mixed urban biowaste: The microbial community shift towards stability.

Anaerobic digestion is applied worldwide to treat food waste (FW) with the aim of obtaining renewable bioenergy by exploiting the methane gas produced. However, there are several problems in practical applications, primarily due to system instability. Although exhaustive knowledge regarding anaerobic microbial community composition has been established, few studies have investigated long-term correlations between microbial consortia, operative conditions and feedstock characteristics. Here, microbial community shifts as a response to feedstock variations were investigated in long-term semi-continuous systems, which were evaluated by an in situ cell detection method and 16S rRNA gene amplicon sequencing. FW digestion showed progressive system instability caused by the inhibition of methanogens, which resulted in volatile fatty acid accumulation and process failure at the low organic loading rate (OLR). Conversely, by co-digesting FW with waste-activated sludge (WAS), a stable process with methane yields of up to 0.27 Nm3 kg-1VSfed for OLR = 1.7 gVS L-1d-1 was achieved. This stabilizing effect was not related to the buffering capacity of WAS, but to its capacity to avoid volatile fatty acid accumulation and falls in pH by overcoming methanogenic activity inhibition. WAS addition promoted the establishment of a stable and active archaeal population in anaerobic co-digestion (AcoD) reactors. The continuous supply of trace elements together with the seeding of microbial functional groups were the main drivers that positively affected process stability.

Anaerobic co-digestion of food waste and waste activated sludge: ADM1 modelling and microbial analysis to gain insights into the two substrates' synergistic effects.

The reasons for the acidification problem affecting Food Waste (FW) anaerobic digestion were explored, combining the outcomes of microbiological data (FISH and CARD-FISH) and process modelling, based on the Anaerobic Digestion Model n°1 (ADM1). Long term semi continuous experiments were carried out, both with sole FW and with Waste Activated Sludge (WAS) as a co-substrate, at varying operational conditions (0.8-2.2 g VS L-1 d-1) and FW / WAS ratios. Acidification was observed along FW mono-digestion, making it necessary to buffer the digesters; ADM1 modelling and experimental results suggested that this phenomenon was due to the methanogenic activity decline, most likely related to a deficiency in trace elements. WAS addition, even at proportions as low as 10% of the organic load, settled the acidification issue; this ability was related to the promotion of the methanogenic activity and the consequent enhancement of acetate consumption, rather than to WAS buffering capacity. The ability of the ADM1 to model processes affected by low microbial activity, such as FW mono-digestion, was also assessed. It was observed that the ADM1 was only adequate for digestions with a high activity level for both bacteria and methanogens (FISH/CARD-FISH ratio preferably >0.8) and, under these conditions, the model was able to correctly predict the relative abundance of both microbial populations, extrapolated from FISH analysis.

Variability of food waste chemical composition: Impact of thermal pre-treatment on lignocellulosic matrix and anaerobic biodegradability.

A comprehensive sustainable Food Waste (FW) management is globally needed in order to reduce the environmental pollution and the financial costs due to FW disposal; anaerobic digestion is considered as one of the best environmental-friendly alternatives to this aim. A deep investigation of the chemical composition of different Food waste types (cooked kitchen waste (CKW), fruit and vegetable scraps (FVS) and organic fraction of municipal solid waste (OFMSW)) is here reported, in order to evaluate their relevant substance-specific properties and their impact on anaerobic biodegradability by means of a sophisticated automatic batch test system. Suitability for a mild thermal pre-treatment (T = 134 °C and p = 3.2 bar) to enhance the biological degradation of hardly accessible compounds was investigated. The pre-treatment affected significantly the carbohydrates solubilisation, and was able in reducing part of the lignocellulosic matrix. Moreover, in mesophilic conditions, the high solubilized sugars content favoured the initial recovery of hydrogen (not consumed by hydrogenotrophic methanogenesis), allowing to newly assess the extent of prompt fermentability. Pre-treatment enhanced hydrogen yields of FVS and OFMSW, with gains up to +50%, while the successive methane production, occurring in the same reactor, resulted affected by the lack of the soluble part of carbohydrates, "subtracted" for H2 production. Only in thermophilic conditions, when no hydrogen in the biogas was detected, pre-treatment of OFMSW significantly increased methane yield (from 0.343 to 0.389 L CH4 g-1 VSfed). A thermal pre-treatment seems the recommended solution in order to reduce part of the recalcitrant lignocellulosic matrix of food waste, to improve energy recovery and to eliminate the extra cost needed for pasteurization.